目的 考察栀子苷磷脂复合物对肠道菌群降解栀子苷的影响,为设计适宜的栀子苷口服制剂提供理论依据。方法 收集新鲜大鼠粪便制备肠道菌群孵育液,将栀子苷、栀子苷物理混合物及其复合物分别与大鼠肠道菌群孵育液在无氧条件下培养,于0、2、4、6、8、12 h取样,采用HPLC测定栀子苷含量,并对制备的栀子苷磷脂复合物进行DSC和X射线衍射表征。结果 在栀子苷和栀子苷磷脂物理混合物组中,前2 h栀子苷不断减少,而2~4 h 时降解加快并于4 h趋于降解完全。而在磷脂复合物组中,6 h内栀子苷含量下降比例为67.2%,8 h时才降解完全。结论 在离体条件下,雄性SD大鼠肠道菌群可以将栀子苷基本降解完全,而制备成磷脂复合物后,栀子苷的降解速率有所下降,物理混合物组中,磷脂并未降低栀子苷的降解速率。
Abstract
OBJECTIVE To investigate the effect of geniposide phospholipid complex on the degradation of geniposide by intestinal microflora, which provides the rationale for the design of an appropriate geniposide dosage form.METHODS The intestinal flora was collected from fresh rat feces. The geniposide and geniposide physical mixtures and their complexes were incubated with the rat intestinal flora in anaerobic conditions, respectively. Samples were taken at 0,2, 4, 6, 8 and 12 h. The content of geniposide was determined by HPLC. The preparative geniposide phospholipid complexes were characterized by DSC and X-ray diffraction.RESULTS In the physical mixture of geniposide and geniposide phospholipids, geniposide continuously decreased in the first 2 h, while the degradation accelerated at 2 to 4 h and completely degraded at 4 h. In the phospholipid complex group, the content of geniposide was decreased by 67.2% within 6 h, and it was completely degraded at 8 h.CONCLUSION The geniposide can be completely degraded in the gut microbiota of male SD rats in vitro. After the preparation of the phospholipid complex, the degradation rate of geniposide was slowed down. In the physical mixture group, the presence of phospholipids does not delay the degradation of jasminoidin.
关键词
栀子苷 /
栀子苷磷脂复合物 /
肠道菌群 /
降解动力学
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Key words
geniposide /
geniposide phospholipid complex /
intestinal flora /
degradation kinetics
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中图分类号：
R965
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